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| HPTLC-DPPH colorimetric-bioautographic image quantification of phenolic antioxidant: taking TBHQ in edible oil and baking foods as an example |
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| DOI： |
| KeyWord:HPTLC DPPH TBHQ cereal and oil food |
| FundProject:国家自然科学基金项目（21804058）；南昌大学食品科学与技术国家重点实验室开放基金项目（SKLF-KF-202001） |
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| Abstract: |
| At present, the common detection methods of phenolic antioxidant TBHQ have the problems of cumbersome pretreatment, long time-consuming and can only determine one sample at a time. Therefore, it is urgent to establish a high-throughput, fast and accurate detection method. A rapid method for screening phenolic antioxidant TBHQ was established by combining high performance thin layer chromatography (HPTLC) separation with reduction discoloration reaction of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and quantitative analysis of image pixels. On this basis, edible oil, bread and cake were taken as representative samples to verify the practical application performance of the proposed method. The results showed that the detection limit of the established method to detect TBHQ was 70 ng/zone (35 mg/kg), the limit of quantification was 140 ng/zone (70 mg/kg), and the limit of quantification was lower than the limit of use (200 mg/kg) specified in GB 2760-2014. Through comparative analysis, the interference of the background matrix coexisting in the sample extract on the target quantity could be ignored, which proved that the method had good selectivity. The target showed good linearity in the range of 150-550 ng/zone (R2=0.989 2). Secondly, several parallel tests (n=3) and the test results of spiked recovery near the specified limit level of national standard proved that the method had good precision and accuracy. In summary, this method has the advantages of strong matrix tolerance, accurate quantification, simpleness and reliableness, and large analytical flux. Therefore, it is particularly suitable for rapid quantitative screening of various antioxidant additives in cereal and oil food, and has the potential and value of becoming a national standard in related fields. |
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