In order to improve the quality of lacquer seed oil, Shaanxi lacquer tree seed was used as raw material to study the effect of lacquer seed kernel pretreatment method on the extraction of lacquer seed oil. The dewaxing conditions of lacquer seed oil were optimized through orthogonal experiment. The effect of the amount of filter aid on the filtration rate of wax crystal was studied, and the physicochemical indexes, compositions of fatty acid, vitamin E and phytosterol in lacquer seed oil were determined. The results showed that the quality of lacquer seed oil extracted from lacquer seed kernel pretreated with hot alkaline water was better, and this process was more suitable for practical production. The optimal dewaxing conditions of lacquer seed oil suitable for large-scale production were obtained as follows: cooling rate 1.0 ℃/h, crystallization temperature 5 ℃ and stirring speed 20 r/min. Adding 2.0% perlite as filter aid, the separation speed of lacquer seed oil and lacquer wax was the fastest. Under the optimal conditions, the dewaxing rate of lacquer seed oil was 95.88%. Lacquer seed oil contained high content of linoleic acid (68.7%), δ-tocopherol (422.78 mg/kg) and phytosterol (3 760.00 mg/kg), and β-fusosterol (386.21 mg/kg) and limonol (138.55 mg/kg) were substances not reported in common vegetable oils. |