| In order to explore plant-derived protein peptides with good antioxidant activities, hemp seed meal was hydrolyzed by papain and neutral protease to obtain hemp seed protein hydrolysate, and four kinds of polypeptide component with different molecular weights were obtained by membrane separation technology. The antioxidant activity of different polypeptide components was evaluated by the determination of DPPH radical scavenging rate and total reducing power. The amino acid sequence of the polypeptide component with the strongest antioxidant activity was analyzed. The results showed that low molecular weight hemp seed polypeptide component had better antioxidant activity, and the antioxidant activity of HP-Ⅳ component (molecular weight <1 kDa) was the strongest. Six peptides with high abundance in HP-Ⅳ component were 364.84,539.77,630.30,662.33,718.90,827.41 Da, and their amino acid sequence were NRDDMRER, ANQLDQFSR, NPEDEFEQLRREGGRG, NHNNQLDLTPR, TVNSYNLPILRF, VSLLDTSNVNNQLDDNPRRFY, respectively. It is suggested that hemp seed meal protein hydrolysates, especially polypeptide component with molecular weight less than 1 kDa, may be used as functional components in the development of antioxidant related functional foods and health care products. |
