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Effect of oil production process on the biologically active ingredients and antioxidant activity of oil-tea camellia seed oil |
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DOI: |
KeyWord:oil-tea camellia seed oil fresh pressing method new aqueous method biologically active ingredient DPPH free radical ABTS free radical |
FundProject:长沙理工大学2020年度“双一流”建设项目(CX2020SS82);郑州市第三批“智汇郑州*1125聚才计划”创新领军人才项目(郑政2018-45号) |
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Abstract: |
In order to understand the effect of oil production process on the biologically active ingredients and antioxidant activity of oil-tea camellia seed oil, oil-tea camellia seeds were used as raw materials and five oil production processes including fresh pressing method, solvent extraction method, new aqueous method, cold pressing method and hot pressing method were used to prepare oil-tea camellia seed oil. The oil extraction rate, fatty acid composition, biologically active ingredients (tocopherol, squalene and polyphenol) content, and scavenging activities on DPPH and ABTS free radicals of five kinds of oil-tea camellia seed oils were comparatively investigated. The results showed that the oil extraction rates of the five oil production processes were all above 73%, of which the solvent extraction method ((96.45±3.02)%) was the highest, the cold pressing method ((73.72±2.76)%) was the lowest, and the fresh pressing method ((82.36±2.24)%), the new aqueous method ((81.91±3.21)%) and the hot pressing method ((80.34±2.09)%) had no significant difference. The oil-tea camellia seed oil produced by five processes had similar fatty acid composition. In terms of α-tocopherol and total tocopherol contents, the oil-tea camellia seed oil produced by new aqueous method had the highest content (α-tocopherol content (263.77±1.58)mg/kg, total tocopherol content (280.55±1.64)mg/kg), the total tocopherol content of oil-tea camellia seed oil produced by fresh pressing method, cold pressing method, and hot pressing method was close (between 233 mg/kg and 244 mg/kg), while the total tocopherol content of oil-tea camellia seed oil produced by solvent extraction method was only (17.10±0.76)mg/kg. In terms of squalene and polyphenol contents, the oil-tea camellia seed oil produced by fresh pressing method was significantly higher than that by other processes, reaching (350.56±7.60)mg/kg and (45.04±4.50)mg/kg respectively. The squalene content ((249.99±3.73)mg/kg) of oil-tea camellia seed oil produced by new aqueous method was the lowest. The polyphenol content ((7.06±0.03)mg/kg) of oil-tea camellia seed oil produced by solvent extraction method was the lowest. In terms of scavenging DPPH free radical and ABTS free radical, the oil-tea camellia seed oil produced by fresh pressing method had the strongest scavenging ability, and that produced by solvent extraction method had the weakest scavenging ability. |
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