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| Lipase-catalyzed preparation of medium-and long-chain triglycerides based on walnut oil |
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| DOI： |
| KeyWord:walnut oil medium-and long-chain triglycerides lipase transesterification ethanol extraction |
| FundProject:国家自然科学基金(31601433) |
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| Abstract: |
| The product with high content of medium-and long-chain triglycerides (MLCT) was prepared by using lipase to catalyze the transesterification of medium-chain triglycerides (MCT) with walnut oil, using molecular distillation to remove the free fatty acid (FFA), and using ethanol to extract the MCT from the product. The enzyme-catalyzed transesterification reaction conditions and ethanol extraction conditions were optimized, and the fatty acid composition and distribution of the product were determined. The results showed that the optimal transesterification reaction conditions were obtained as follows: Novozym 435 dosage 10%, molar ratio of MCT to walnut oil 2∶ 1, reaction temperature 55 ℃ and reaction time 9 h. After reaction under these conditions and removal of FFA with molecular distillation, the contents of MLCT, MCT and long-chain triglycerides (LCT) were 76.2%, 14.3% and 9.5%, respectively. The optimal ethanol extraction conditions were obtained as follows: ethanol volume fraction 85%, and ratio of substrate (the transesterification product after molecular distillation) to ethanol solution 1∶ 3. Under these conditions, the contents of MLCT, MCT and LCT were 85.4%, 3.7% and 10.8%, respectively, and the product yield was 80.8%. This product with high content of MLCT was rich in linoleic acid and linolenic acid, and the content ratio of linoleic acid to linolenic acid was 5.8, which could be used as a source of oil to supplement essential fatty acids for patients with digestive system defects. |
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