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Enrichment of α-eleostearic acid from bitter gourd seed oil by urea adduction fractionation
  
DOI：
KeyWord:bitter gourd seed oil  conjugated linolenic acid  α-eleostearic acid  urea adduction fractionation  enrichment and purification
FundProject:湖北省果蔬精深加工及营养功能食品开发企校联合创新中心（whpu-2020-kj-304）
Author NameAffiliation
SHUAI Xiaoyan .Modern Industry School of Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China 
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Abstract:
      Using bitter gourd seed oil extracted by supercritical CO2 as raw material, the mixed fatty acid was obtained by saponification and acid hydrolysis, and then the α-eleostearic acid was enriched by urea adduction fractionation. Based on single factor experiment, the enrichment conditions were optimized by orthogonal experiment. The results showed that the optimal process for enriching α- eleostearic acid from bitter gourd seed oil were obtained as follows: with 95% ethanol as urea solvent, mass ratio of mixed fatty acid to urea 1∶ 3, mass ratio of urea to 95% ethanol 1∶ 5, adduction temperature 4 ℃ and adduction time 24 h. Under these conditions, the purity of α- eleostearic acid increased from 2883% (in bitter gourd seed oil ) to 60.03%, the total relative content of conjugated linolenic acid in the product reached 71.28%.
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