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| Optimization of preparation process of immobilized Rhizomucor miehei lipase and its enzymatic properties |
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| DOI： |
| KeyWord:Rhizomucor miehei lipase immobilization sodium alginate enzymatic property embedding-crosslinking method |
| FundProject:湖南省重点领域研发计划项目（2019NK2091）；湖南省研究生科研创新项目（CX20190507） |
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| Abstract: |
| With sodium alginate and sodium carboxymethyl cellulose(CMC) as the compound carrier, glutaraldehyde as the crosslinking agent, the process conditions of preparing immobilized Rhizomucor miehei lipase(RML) by embedding- crosslinking method was optimized, and its enzymatic properties were analyzed. The results showed that the optimal process conditions of preparing immobilized RML were obtained as follows: sodium alginate mass fraction 2.5%, CMC mass fraction 1.5%, enzyme concentration 800 U/mL, CaCl2 mass fraction 5%, glutaraldehyde mass fraction 0.03% and crosslinking immobilization time 30 min. Under these conditions, the enzyme activity of immobilized RML was 245.58 U/g. Moreover, the immobilized enzyme was performed better in pH stability and thermal stability than free lipase. The addition of crosslinking agent glutaraldehyde could improve the operation stability and storage stability of immobilized lipase, and after recycling 7 times and storing at 4 ℃ for 7 weeks, the relative activity of immobilized RML still kept at 57.39% and 61.89%, respectively. Thus, the immobilized RML prepared by embedding-crosslinking method had better stability and adaptability and laid the foundation for industrial production of enzymatic esterification deacidification. |
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