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| Optimization of extraction of Nannochloropsis sp. lipid with solvent and its lipidomics analysis |
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| DOI： |
| KeyWord:Nannochloropsis sp. lipid eicosapentaenoic acid ethanol extraction lipidomics |
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| Abstract: |
| The extraction of Nannochloropsis sp. lipid by solvent was studied.The effects of seven systems including chloroform-methanol(volume ratio 1∶?1), chloroform, isopropanol, petroleum ether, n-hexane-ethanol (volume ratio 1∶?1), n-hexane and ethanol on the yield and EPA content of Nannochloropsis sp. lipid were compared. Ethanol was determined as the extraction solvent. The effects of ethanol volume fraction, material-to-liquid ratio, extraction temperature and extraction time on the extraction of Nannochloropsis sp. lipid was studied by single factor experiment and the process conditions were optimized by orthogonal experiment. Moreover, the lipidomics of the Nannochloropsis sp. lipid were analyzed by UPLC-Q-TOF-MS. The results showed that the optimal extraction conditions were obtained as follows:ethanol volume fraction 80%, material-to-liquid ratio 1∶?17.5, extraction temperature 65?℃, and extraction time 2 h. Under these conditions, the yield of Nannochloropsis sp.lipid was (15.87±0.21)%, and EPA content was (14.49±014)g/100 g. Seventeen kinds of lipids were determined in Nannochloropsis sp.lipid, in which only twelve kinds of lipid contained EPA. The lipids containing EPA accounted for 45.24% of the total lipids. |
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