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| Preparation and evaluation of wound healing-promoting activity of peptides from housefly pupa |
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| DOI： |
| KeyWord:housefly pupa peptides enzymolysis method cell proliferation cell migration wound healing |
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| Author Name | Affiliation | | ZHANG Sichen1, SUN Tingting1, ZHAO Zhimin1,2, HE Guorui2, YANG Depo1,2 | 1.School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China
2.Guangdong
Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China |
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| Abstract: |
| In order to determine the process conditions for preparing peptides from housefly pupa (PHP) using enzymolysis method and evaluate the wound healing-promoting activity of PHP, PHP were prepared with enzymolysis methods using pupa of Musca domestica L. as the raw material. The single factor experiment was designed to optimize the preparation process of PHP and the wound healing-promoting activity of PHP was studied at the cellular level. The results showed that among the PHP prepared with ten proteases, the yield of PHP prepared with pepsin was the highest, followed by flavourzyme hydrolysis (the peptide was referred to as PHF). In addition, PHF showed the highest peptides content and degree of hydrolysis. The results of single factor experiment displayed that PHF presented the highest yield and degree of hydrolysis under the conditions of temperature 60 ℃, enzyme dosage 8 000 U/g, and time 4 h. Besides, PHP prepared with different proteases had different degrees of promoting proliferation and migration of HaCaT cells, among them, PHF (molecular weight mainly distributed between 500 Da and 1 300 Da) exhibited the best wound healing-promoting activity. Meantime, PHF significantly promoted the expression of transforming growth factor TGF-β1 and inflammatory cytokines TNF-α, IL-6 in RAW264.7 cells. In conclusion, the enzymolysis method with flavourzyme contributes to prepare PHP with high-yield, high-peptides content, and high-degree of hydrolysis, and PHF has the potential to promote wound healing. |
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