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| Inhibition of phytosterols on lipopolysaccharide-induced inflammation in mice |
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| DOI： |
| KeyWord:phytosterols lipopolysaccharide inflammation iNOS NF-κB SHP-1 alternative antibiotics |
| FundProject: |
| Author Name | Affiliation | | ZHAO Yunbo1, WANG Ningjing1, HU Yongyuan1, NIU Yongjie1, MENG Yonghong2 | 1. Shaanxi Healthful Bio-engineering Co. , Ltd. , Yangling 712199, Shaanxi, China； 2. College of Food Engineering and Nutritional Science，Shaanxi Normal University, Xi’an 710119, China |
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| Abstract: |
| Taking mice prefed with phytosterols as the research object, lipopolysaccharide was used to induce inflammation in mice, and the preventive effects of different dosages of phytosterols on inflammation were studied. The results showed that compared with lipopolysaccharide group, inflammatory factors interleukin-1β（IL-1β）, interleukin-6 (IL-6) and tumor necrosis factor α（TNF- α）in serum of mice in phytosterol group decreased significantly (P<0.05) or extremely significantly (P<0.01). The inflammatory lesions of liver and spleen were significantly improved. The secretion of nitric oxide synthase (iNOS) in liver and spleen of mice in phytosterol groups decreased significantly (P<0.05) or extremely significantly (P<0.01). At the protein level, the expressions of nuclear transcription factor κB（NF- κB) p65 protein and its phosphorylated protein decreased significantly(P<0.05) or extremely significantly (P<0.01). At the same time, compared with the lipopolysaccharide group, the protein tyrosine phosphatase-1 (SHP-1) in liver and spleen of mice prefed with phytosterols increased extremely significantly (P<0.01) after inflammation. It was speculated that after the expression of negative regulator SHP-1 was up-regulated, NF-κB activation was inhibited, which reduced the expression level of iNOS and inhibited the downstream inflammatory factors IL-1β、 IL-6 and TNF- α, playing a role in reducing the invasion of inflammation to body organs. Therefore, phytosterols have a significant effect in reducing inflammation in mice and are of great significance in preventing infection in cultured animals. |
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