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| Optimization of detection methods for sesamin,sesamolin and asarinin in sesame oil |
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| KeyWord:HPLC sesame oil sesamin sesamolin asarinin |
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| Abstract: |
| The content of sesamin, sesamolin and asarinin in sesame oil was simultaneously analyzed by organic solvent methanol extraction and HPLC method. In order to improve the analysis quality and accuracy, the dosage of methanol extraction solvent and the time of ultrasound-assisted extraction were optimal, and the detection method was investigated. The results showed that the optimal extraction conditions were methanol dosage 10 mL（0.1 g sesame oil）, ultrasonic time 2 min. The sesamin, sesamolin and asarinin had good linearity within the ranges of 4-100 mg/L, 4-100 mg/L and 1-40 mg/L, respectively, and their correlation coefficients were 0.999 5, 0.999 6 and 0.999 2, respectively. The LOD of sesamin, sesamolin and asarinin were 0.03, 0.02 mg/g and 0.02 mg/g, respectively, and their LOQ were 0.06, 0.06 mg/g and 0.04 mg/g, respectively. The recoveries of sesamin, sesamolin and asarinin were 87.17%-92.59%, 92.71%-102.24% and 95.66%-10893%, respectively, with RSD all less than 5%. The stability, accuracy and precision of the method met the testing requirements. In conclusion, the method has advantages of simple operation, short time-consuming (only 30 min to complete an analysis), low cost, high stability, etc., and can be effectively applied to the analysis and detection of three lignan components in sesame oil. |
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