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| Enzymatic synthesis of medium and long-chain structured lipids rich in docosahexaenoic acid at sn-2 position |
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| DOI： |
| KeyWord:docosahexaenoic acid medium and long-chain structured lipid monoglyceride lipase alcoholysis esterification |
| FundProject: |
| Author Name | Affiliation | | JIANG Xuan, YANG Yao, XU Xiuli, CHAO Zhonghao, ZHANG Shiqun, CHENG Yang, ZOU Xiaoqiang | School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China |
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| Abstract: |
| Medium and long-chain structured lipids (MLSL) rich in docosahexaenoic acid (DHA) at sn-2 position were prepared by a two-step enzymatic method. In the first step, Lipozyme 435 was used to catalyze the alcoholysis of DHA oil, and monoglyceride (MAG) rich in DHA was obtained by solvent extraction. Then the lipase-catalyzed esterification of MAG and capric acid (CA) was employed to obtain MLSL rich in DHA at sn-2 position. The results showed that the optimal enzymatic esterification conditions were obtained as follows: vacuum 0.05 MPa, Lipozyme TL IM dosage 8%(based on the mass of substrates), ratio of MAG to CA 1∶3, reaction time 9 h, and reaction temperature 25℃. Under the optimal conditions, the triglycerides (TAG)content in the product was 96.55%, among which the DHA accounted for 40.04% of the total fatty acids and 72.15% of the sn-2 fatty acids. In the purfied product of TAG, the content of total MLSL was 99.14% and the MLSL with DHA accounted for 67.69%. |
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