|
| Physicochemical properties and biological activity of broad bean protein hydrolysate obtained by membrane separation technology |
| |
| DOI： |
| KeyWord:broad bean protein enzymatic hydrolysate membrane separation in vitro antioxidation α-glucosidase |
| FundProject: |
| Author Name | Affiliation | | DENG Yongrong1, HAN Lijuan1,2, YANG Xijuan1,2, DANG Bin1,2, ZHOU Wen2, ZHANG Xue1, DAI Yunli1 | 1.College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China； 2.Qinghai Tibetan Plateau Agricultural Processing Key Laboratory, Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, China |
|
| Hits: 1068 |
| Download times: 870 |
| Abstract: |
| Fresh broad beans were used as raw materials, broad bean protein was extracted by alkali solution and acid precipitation. Four different proteases were used for single enzyme or double enzyme hydrolysis of broad bean protein, and the best two enzymes were selected for the complex enzymatic hydrolysis of broad bean protein by comparing the hydrolysis degree and polypeptide yield of broad bean protein. Then the broad bean protein hydrolysate (BBPHs) were fractionated by membrane separation into five fractions of BBPHs-Ⅰ(<1 kDa), BBPHs-Ⅱ(1-3 kDa), BBPHs-Ⅲ(3-5 kDa), BBPHs-Ⅳ(5-10 kDa), BBPHs-Ⅴ(>10 kDa).The amino acid composition, UV and IR spectra of the five fractions were analyzed, and their biological activities were characterized by in vitro antioxidant activity and α-glucosidase inhibition rate. The results showed that pineapple protease and papain were selected for the complex enzymatic hydrolysis of broad bean protein.The total amino acid content of broad bean protease hydrolysate below 10 kDa after membrane separation increased compared with that without membrane separation,and the hydrophobic amino acid contents of BBPHs-Ⅱ, BBPHs-Ⅲ and BBPHs-Ⅳ were higher.In addition, BBPHs-Ⅲ had the highest content of total amino acid, essential amino acid, hydrophobic amino acid and aromatic amino acid with 65.304%, 19.222%, 20.762% and 8.769% respectively.Protein hydrolysate components with different molecular weights showed certain in vitro antioxidant capacity.The ABTS free radical scavenging rate of the BBPHs-Ⅳ could reach (27.89±0.01)%,DPPH free radical scavenging rate of the BBPHs-Ⅱ could reach (57.70±0.00)% at 10 mg/mL mass concentration.When the mass concentration ranged from 2 mg/mL to 32 mg/mL, the α-glucosidase inhibitory activities of different molecular weight broad bean hydrolysates showed a dose-dependent relationship. BBPHs-Ⅱ, BBPHs-Ⅲ and BBPHs-Ⅳ showed good α-glucosidase inhibitory activities,and BBPHs-Ⅲ possessed the best α-glucosidase inhibition rate 〔(86.56±1.23)%〕 at 32 mg/mL mass concentration. Therefore, the small molecular weight broad bean protein hydrolysate obtained by membrane separation had higher antioxidant activity and α-glucosidase inhibitory activity，and had good development and application prospects. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
