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| Determination of natural vitamin A in fish liver by liquid chromatography |
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| DOI： |
| KeyWord:fish liver vitamin A extraction method detection method |
| FundProject: |
| Author Name | Affiliation | | HUANG Wenting1, CAO Wenhong1,2,3,4,5,6,7, ZHANG Chaohua1,2,3,4,5,6,7, QIN Xiaoming1,2,3,4,5,6,7, GAO Jialong1,2,3,4,5,6,7, ZHENG Huina1,2,3,4,5,6,7 | 1.College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China
2.National Research and Development Branch Center for Shellfish Processing, Zhanjiang 524088, Guangdong, China
3.Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, Guangdong, China
4.Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, Guang dong, China
5.Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, Guangdong, China
6.Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088,Guangdong,China
7. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China |
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| Abstract: |
| In order to accurately determine the content of vitamin A (vitamin A1, vitamin A2) in fish liver, sample pretreatment methods (water bath saponification, room temperature saponification, direct extraction) and detection methods (normal phase chromatography, reversed phase chromatography) were screened and applied to the determination of vitamin A in the liver of nine economic fish species. The results showed that vitamin A1 and vitamin A2 were separated effectively by reversed phase chromatography and showed good linear relationship within their respective linear ranges (R2 > 0.99); the content of vitamin A extracted by water bath saponification was significantly higher than that by room temperature saponification and direct extraction (p<0.05), and its average recoveries of vitamin A1 and vitamin A2 were 104.52% and 90.94%, respectively. Except for the freshwater snakehead and big mouth bass, the total content of vitamin A in the livers of other freshwater fishes and marine fishes was more than 200 μg/100 g, and the total content of vitamin A in the liver of marine giant grouper was the highest, reaching 14 413.78 μg/100 g. The water bath saponification method combined with reversed phase chromatography has good precision and is suitable for the determination of vitamin A in fish liver. |
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