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| Immobilization of partial glyceride lipase Lipase G50 and its application in deacidification of high acid value rice bran oil |
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| DOI： |
| KeyWord:partial glycerdie lipase Lipase G50 immobilization high acid value rice bran oil enzymatic deacidification |
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| Abstract: |
| The immobilization of partial glyceride lipase Lipase G50 and its catalytic deacidification of high acid value rice bran oil were studied. The results showed that compared with the other four resins, resin ECR8285 had the best immobilization effect on Lipase G50. When the enzyme loading amount was 40 mg/g, the esterification activity and the specific activity of immobilized Lipase G50 were 518.66 U/g and 15.65 U/mg, respectively. The optimal deacidification conditions catalyzed by immobilized Lipase G50 were obtained as follows: molar ratio of ethanol to free fatty acid in high acid value rice bran oil 2∶1, enzyme dosage 40 U/g, reaction temperature 40℃ and reaction time 6 h. Under the optimal conditions, the acid value of high acid value rice bran oil decreased from 62.14 mgKOH/g to 0.12 mgKOH/g. At the same time, immobilized Lipase G50 showed excellent operational stability in the deacidification of high acid value rice bran oil, with esterification activity of 509.22 U/g after 10 batches of continuous use. The deacidified rice bran oil was purified by molecular distillation, and its acid value and peroxide value were 0.19 mgKOH/g and 2.16 mmol/kg, respectively, which reached the standard of first-grade rice bran oil in GB/T 19112-2003. So immobilized Lipase G50 has a promising future in the field of oil deacidification. |
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