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| Optimization of extraction of Gardenia jasminoides oil by aqueous enzymatic method and its fatty acid composition |
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| DOI： |
| KeyWord:Gardenia jasminoides fruit Gardenia jasminoides oil aqueous enzymatic method uniform design fatty acid composition |
| FundProject: |
| Author Name | Affiliation | | CAO Qiuxia1,2, LIANG Wenbin1, YANG Yan2,3, TANG Yuxi3,TANG Jie3, LI Lei3, HUANG Feng4 | 1.College of Life Science and Technology, Central South Forestry University of Science and Technology,Changsha 410004, China
2.State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China
3.Hunan Academy of Forestry,Changsha 410004, China
4.Baixiang Forestry Workstation of Yueyang County Forestry Bureau, Yueyang 414100, Hunan, China |
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| Abstract: |
| In order to promote the development and utilization of Gardenia jasminoides, With the mature fruit of Gardenia jasminoides as raw material, the oil was extracted by aqueous enzymatic method. The effects of enzyme species, enzyme dosage, enzymatic hydrolysis pH, enzymatic hydrolysis temperature, liquid-material ratio and enzymatic hydrolysis time on the yield of Gardenia jasminoides oil were studied by single factor experiment. The process parameters were optimized by uniform design on the basis of single factor experiment. The fatty acid composition of Gardenia jasminoides oil extracted by different enzymes was analyzed. The results showed that the optimal process conditions were obtained as follows: with neutral protease for enzymolysis, enzyme dosage 0.7%, liquid-material ratio 3∶1, enzymatic hydrolysis pH 7, enzymatic hydrolysis temperature 60℃ and enzymatic hydrolysis time 7 h. Under these conditions, the yield of Gardenia jasminoides oil was 7.27%, which was 117.66% higher than that of control(3.34%). The linoleic acid content in Gardenia jasminoides oil was the highest, exceeding 56%, and unsaturated fatty acids content was about 80%. There was no significant difference in the fatty acid composition and content of Gardenia jasminoides oils extracted by different enzymes. |
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