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| Optimization of aqueous enzymatic extraction of red melon seed kernel oil and its volatile components analysis |
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| KeyWord:aqueous enzymatic method red melon seed kernel oil volatile component |
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| Abstract: |
| In order to obtain the optimal process conditions of extracting oil from red melon seeds with aqueous enzymatic method and understand the changes of volatile components of the red melon seed kernel oil during storage, with the extraction rate as the index, the extraction process conditions of red melon seed kernel oil were optimized by single factor experiment and orthogonal experiment, and the volatile components of red melon seed kernel oil stored for 0 d and 1 year (stored in a refrigerator at 4℃) were analyzed by HS-GC-IMS. The results showed that the optimal process conditions for aqueous enzymatic extraction of red melon seed kernel oil were obtained as follows: solid-liquid ratio 1∶5, enzyme dosage of alkali protease 5%, enzymatic hydrolysis temperature 50℃, enzymatic hydrolysis time 3 h, and pH 10. Under the optimal conditions, the extraction rate of red melon seed kernel oil was 75.68%. There were 25 main volatile components in the red melon seed kernel oil, and there were differences in the content of volatile components in red melon seed kernel oil under different storage time, among which 3-methyl-1-butanol and 3-hydroxyl-2-butanone could be preliminarily identified as a marker of volatile components in fresh red melon seed kernel oil. In conclusion, the aqueous enzymatic extraction of red melon seed kernel oil can obtain higher oil extraction rate, and the storage time affects the composition and content of volatile components in red melon seed kernel oil. |
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