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| Preparation of L-α-glycerylphosphorylethanolamine by hydrolysis of soybean phospholipids powder catalyzed with Rhizopus chinensis |
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| DOI： |
| KeyWord:Rhizopus chinensis soybean phospholipids powder L-α-glycerylphosphorylethanolamine |
| FundProject: |
| Author Name | Affiliation | | LI Hong1, LI Ziyi1, ZHANG Lixia2, ZHANG Kangyi2, SHEN Ruiling1, WEI Tao1 | 1.College of Food and Biological Engineering,Zhengzhou University of Light Industry, Zhengzhou 450000, China
2.Research Center of Agricultural Products Processing, Henan Academy of Agriculture Sciences, Zhengzhou 450002, China |
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| Abstract: |
| To investigate the feasibility of L-α-glycerylphosphorylethanolamine (L-α-GPE) preparation from hydrolysis of soybean phospholipids powder with Rhizopus chinensis as catalyst, L-α-GPE was prepared by Rhizopus chinensis-catalyzed hydrolysis of soybean phospholipids powder. The effects of pH, soybean phospholipid powder mass concentration, reaction temperature, CaCl2 mass concentration and enzyme concentration on the yield of L-α-GPE were investigated by single-factor experiment, and the structure of the purified products were verified by infrared spectroscopy and UPLC-Q-TOF-MS. The results showed that the yield of L-α-GPE was 93.8% under the conditions of addition of final mass concentration of CaCl2 2 mg/mL and final concentration of Rhizopus chinensis 30 U/mL in soybean phospholipids powder mass concentration 5 mg/mL, phosphate buffer solution pH 7， reaction time 15 h, and reaction temperature 45℃. After purification， a light yellow viscous liquid product with a purity of 99.2% was obtained, which was verified as L-α-GPE by structural verification. |
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