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| Effects of natural antioxidant on in vitro antioxidant activity of crocodile oil |
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| DOI： |
| KeyWord:crocodile oil natural antioxidant antioxidant activity free radical |
| FundProject: |
| Author Name | Affiliation | | FU Lijun1,2 ,WANG Haiyan3 ,ZHENG Yahong1,2，YANG Lei1,2，LI Hualiang4，DING Yumei4 | 1.Fujian Provincial Key Laboratory of Ecology-Toxicological Effects ＆ Control for Emerging Contaminants，Putian 351100,Fujian,China
2.Ecological Environment and Its Information Atlas Fujian Key Laboratory of Colleges and Universities, Putian 351100,Fujian,China
3.New Engineering Industry College, Putian University, Putian 351100,Fujian,China
4.Fujian Alligator Dragon Industrial Co. , Ltd. , Xiamen 361026,Fujian,China |
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| Abstract: |
| In order to screen natural antioxidant and improve the in vitro antioxidant activity of crocodile, the Schaal oven method was used to compare the antioxidant effects of three common natural antioxidants tea polyphenols (TP), VE and phytic acid on refined crocodile oil, and the antioxidant with stronger antioxidant capacity was selected. Then the DPPH radical, hydroxyl radical (·OH), superoxiden anion radical (O-2·) scavenging ability, reducing capacity and Fe2+ chelating capacity of crocodile oil added with selected antioxidant was evaluated by in vitro antioxidant method using added antioxidants and VC as positive control. The results showed that the antioxidant effect of TP was the strongest, followed by VE, and the antioxidant effect of phytic acid was the lowest.The scavenging ability of crocodile oil to DPPH free radical, ·OH,O-2· , and the reducing ability and Fe2+ chelating ability increased with the increase of crocodile oil mass concentration.The in vitro antioxidant capacity of crocodile oil was significantly enhanced by the addition of 0.02% TP. The IC50 of crocodile oil with TP against DPPH radicals, ·OH、O-2· were 0.874,0.064 mg/mL and 0.96 mg/mL, respectively, which were lower than those of crocodile oil (5.448, 0.684, 4.53 mg/mL), and the reducing ability and Fe2+ chelating ability were higher than those of crocodile oil. In summary, TP can effectively enhance the in vitro antioxidant activity of crocodile oil. |
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