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| Optimization of culture conditions for fatty acid production of recombinant Corynebacterium glutamicum G-BC by response surface methodology |
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| DOI： |
| KeyWord:recombinant Corynebacterium glutamicum culture condition fatty acid acetyl-CoA carboxylase α subunit |
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| Abstract: |
| Acetyl-CoA carboxylase is a key enzyme regulating fatty acid synthesis in Corynebacterium glutamicum. In order to study the effect of acetyl-CoA carboxylase α subunit (accBC) gene on fatty acid synthesis,the recombinant Corynebacterium glutamicum G-BC(expression strain of accBC) was used as raw material, the effects of inducer(isopropyl-β-D-thiogalactopyranoside,IPTG) concentration, culture time and culture temperature on the yield of fatty acid were studied. Moreover, the culture conditions for fatty acid production of recombinant Corynebacterium glutamicum G-BC were optimized by response surface methodology. The fatty acid yield of the original strain and the recombinant strain was also compared.The results showed that the optimal culture conditions were obtained as follows: inducer concentration 1 mmol/L, culture time 20 h, and culture temperature 32℃. Under these conditions, the yield of fatty acid was 34.56 mg/g（based on dry weight of bacteria）, which was 1.68 times higher than original strain. The accBC can promote the synthesis of fatty acid by Corynebacterium glutamicum. |
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