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| Enzymatic degumming of cold pressed rapeseed oil with phospholipase Lecitase Ultra and phospholipase C |
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| DOI： |
| KeyWord:phospholipase Lecitase Ultra phospholipase C cold pressed rapeseed oil degumming |
| FundProject: |
| Author Name | Affiliation | | MA Yunrui1, ZHOU Li1, GAO Pan1,2, HU Chuanrong1,2, LUO Zhi1,2, HE Dongping1,2 | 1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430000, China
2.Key Laboratory of Deep Processing of Major Grain and Oil of Education, Wuhan 430023, China |
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| Abstract: |
| In order to improve the degumming efficiency, using crude cold pressed rapeseed oil as raw material, phospholipid content as an indicator, the degumming of cold pressed rapeseed oil with complex enzyme of phospholipase Lecitase Ultra and phospholipase C was investigated. The effects of phospholipase Lecitase Ultra reaction time, phospholipase C reaction time, water dosage, phospholipase Lecitase Ultra dosage, phospholipase C dosage, and citric acid dosage on the phospholipid content were studied with single factor experiment, and the degumming conditions were optimized by response surface methodology. The physicochemical indicators of the degummed oil obtained under the optimized degumming conditions were tested and compared with the national standard first-class pressed rapeseed oil. The results showed that the optimal conditions for the enzymatic degumming of cold pressed rapeseed oil with phospholipase Lecitase Ultra and phospholipase C were obtained as follows: phospholipase Lecitase Ultra dosage 33 mg/kg, phospholipase Lecitase Ultra reaction time 90 min, phospholipase C dosage 65 mg/kg, phospholipase C reaction time 60 min, water dosage 33 mL/kg, citric acid dosage 1.2 mL/kg. Under the optimal conditions, the phospholipid content was 2.3 mg/kg, and the peroxide value and acid value of the degummed oil reached the national standard of first-class pressed rapeseed oil. In conclusion, the compound degumming effect of phospholipase Lecitase Ultra and phospholipase C is better, and the optimized process conditions can be used for rapeseed oil degumming. |
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