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| 磷脂酶Lecitase Ultra和磷脂酶C复合对冷榨菜籽油脱胶的影响 |
| Enzymatic degumming of cold pressed rapeseed oil with phospholipase Lecitase Ultra and phospholipase C |
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| DOI： |
| 中文关键词: 磷脂酶Lecitase Ultra 磷脂酶C 冷榨菜籽油 脱胶 |
| 英文关键词:phospholipase Lecitase Ultra phospholipase C cold pressed rapeseed oil degumming |
| 基金项目: |
| Author Name | Affiliation | | MA Yunrui1, ZHOU Li1, GAO Pan1,2, HU Chuanrong1,2, LUO Zhi1,2, HE Dongping1,2 | 1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430000, China
2.Key Laboratory of Deep Processing of Major Grain and Oil of Education, Wuhan 430023, China |
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| 中文摘要: |
| 为了提高脱胶效率，以冷榨菜籽原油为原料，磷脂含量为指标，采用磷脂酶Lecitase Ultra和磷脂酶C复合酶法对冷榨菜籽油进行脱胶。采用单因素试验考察磷脂酶Lecitase Ultra反应时间、磷脂酶C反应时间、加水量、磷脂酶Lecitase Ultra添加量、磷脂酶C添加量、柠檬酸溶液添加量对脱胶油磷脂含量的影响，并通过响应面法优化脱胶条件。对优化的脱胶条件下所得到的脱胶油的理化指标进行了检测，并与国标一级压榨菜籽油进行了比较。结果表明：磷脂酶Lecitase Ultra和磷脂酶C对冷榨菜籽油进行酶法脱胶的最佳工艺条件为磷脂酶Lecitase Ultra添加量33 mg/kg，磷酯酶Lectase Ultra反应时间90 min，磷脂酶C添加量65 mg/kg，磷脂酶C反应时间60 min，加水量33 mL/kg，柠檬酸溶液添加量1.2 mL/kg；在优化条件下脱胶，脱胶油中磷脂含量为2.3 mg/kg，脱胶油的过氧化值和酸值均达到一级压榨菜籽油的国家标准。综上，磷脂酶Lecitase Ultra和磷脂酶C复合脱胶效果较好，所优化的工艺条件可用于菜籽油的脱胶。 |
| 英文摘要: |
| In order to improve the degumming efficiency, using crude cold pressed rapeseed oil as raw material, phospholipid content as an indicator, the degumming of cold pressed rapeseed oil with complex enzyme of phospholipase Lecitase Ultra and phospholipase C was investigated. The effects of phospholipase Lecitase Ultra reaction time, phospholipase C reaction time, water dosage, phospholipase Lecitase Ultra dosage, phospholipase C dosage, and citric acid dosage on the phospholipid content were studied with single factor experiment, and the degumming conditions were optimized by response surface methodology. The physicochemical indicators of the degummed oil obtained under the optimized degumming conditions were tested and compared with the national standard first-class pressed rapeseed oil. The results showed that the optimal conditions for the enzymatic degumming of cold pressed rapeseed oil with phospholipase Lecitase Ultra and phospholipase C were obtained as follows: phospholipase Lecitase Ultra dosage 33 mg/kg, phospholipase Lecitase Ultra reaction time 90 min, phospholipase C dosage 65 mg/kg, phospholipase C reaction time 60 min, water dosage 33 mL/kg, citric acid dosage 1.2 mL/kg. Under the optimal conditions, the phospholipid content was 2.3 mg/kg, and the peroxide value and acid value of the degummed oil reached the national standard of first-class pressed rapeseed oil. In conclusion, the compound degumming effect of phospholipase Lecitase Ultra and phospholipase C is better, and the optimized process conditions can be used for rapeseed oil degumming. |
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