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| 响应面试验优化碱提酸沉法提取微胚乳玉米蛋白工艺及其组成分析 |
| Optimization of alkali-extraction and acid-precipitation extraction of micro-endosperm corn protein by response surface methodology and its composition analysis |
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| DOI： |
| 中文关键词: 微胚乳玉米 玉米蛋白 碱提酸沉法 分子质量 氨基酸组成 |
| 英文关键词:micro-endosperm corn corn protein alkali-extraction and acid-precipitation molecular weight amino acid composition |
| 基金项目: |
| Author Name | Affiliation | | WEI Ping, ZHANG Yayuan, YOU Xiangrong, DENG Jingyou, ZHOU Kui, YANG Zhaoxing | Guangxi Engineering Technology Research Center of Banana Preservation and Processing, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China |
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| 中文摘要: |
| 对碱提酸沉法提取微胚乳玉米蛋白的工艺条件进行优化，并分析其分子质量分布和氨基酸组成。以微胚乳玉米为原料，采用碱提酸沉法提取其中的蛋白质，以玉米蛋白得率为指标，采用单因素试验考察了料液比、提取pH、提取温度和提取时间的影响，采用响应面试验进一步对微胚乳玉米蛋白提取的工艺条件进行了优化。采用SDS-PAGE和氨基酸组成分析比较了最优条件下提取的微胚乳玉米蛋白与普通玉米蛋白的差异。结果表明:微胚乳玉米蛋白的最优提取工艺条件为料液比1∶15、提取pH 11.5、提取温度50℃、提取时间60 min，在此条件下微胚乳玉米蛋白得率为20.40%;微胚乳玉米蛋白与普通玉米蛋白的分子质量均小于60 kDa，但在15～20 kDa之间二者出现的条带略有不同，两种蛋白中氨基酸种类齐全，谷氨酸、亮氨酸、精氨酸、天门冬氨酸含量较高，微胚乳玉米蛋白的必需氨基酸含量占氨基酸总量的36.15%，略高于普通玉米蛋白的35.44%。该研究可为微胚乳玉米蛋白精深加工及其应用提供一定科学依据。 |
| 英文摘要: |
| The extraction conditions of micro-endosperm corn protein by alkali-extraction and acid-precipitation method were optimized, and its molecular weight distribution and amino acid composition were analyzed. With micro-endosperm corn protein yield as the index, the effects of ratio of material to liquid, extraction pH, extraction temperature and extraction time were studied by single factor experiment, and then the extraction conditions were optimized by response surface methodology. The difference between micro-endosperm corn protein and normal corn protein obtained under the optimal condtions were compared by SDS-PAGE and amino acid composition analysis. The results showed that the optimal extraction conditions for micro-endosperm corn protein were obtained as follows: ratio of material to liquid 1∶15, extraction pH 11.5, extraction temperature 50℃ and extraction time 60 min. Under the optimal conditions, the yield of micro-endosperm corn protein was 20.40%. The molecular weight of micro-endosperm corn protein and normal corn protein were both less than 60 kDa, but the bands appearing between 15 kDa and 20 kDa were slightly different. The two corn protein contained almost all kinds of amino acids with glutamic acid, leucine, arginine and aspartate being the predominant amino acids, and the essential amino acid content of micro-endosperm corn protein was 36.15%, which was slightly higher than that of normal corn protein(35.44%). This study can provide a certain scientific basis for the deep processing and application of micro-endosperm corn protein. |
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