|
| Optimization of alkali-extraction and acid-precipitation extraction of micro-endosperm corn protein by response surface methodology and its composition analysis |
| |
| DOI： |
| KeyWord:micro-endosperm corn corn protein alkali-extraction and acid-precipitation molecular weight amino acid composition |
| FundProject: |
| Author Name | Affiliation | | WEI Ping, ZHANG Yayuan, YOU Xiangrong, DENG Jingyou, ZHOU Kui, YANG Zhaoxing | Guangxi Engineering Technology Research Center of Banana Preservation and Processing, Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China |
|
| Hits: 1174 |
| Download times: 832 |
| Abstract: |
| The extraction conditions of micro-endosperm corn protein by alkali-extraction and acid-precipitation method were optimized, and its molecular weight distribution and amino acid composition were analyzed. With micro-endosperm corn protein yield as the index, the effects of ratio of material to liquid, extraction pH, extraction temperature and extraction time were studied by single factor experiment, and then the extraction conditions were optimized by response surface methodology. The difference between micro-endosperm corn protein and normal corn protein obtained under the optimal condtions were compared by SDS-PAGE and amino acid composition analysis. The results showed that the optimal extraction conditions for micro-endosperm corn protein were obtained as follows: ratio of material to liquid 1∶15, extraction pH 11.5, extraction temperature 50℃ and extraction time 60 min. Under the optimal conditions, the yield of micro-endosperm corn protein was 20.40%. The molecular weight of micro-endosperm corn protein and normal corn protein were both less than 60 kDa, but the bands appearing between 15 kDa and 20 kDa were slightly different. The two corn protein contained almost all kinds of amino acids with glutamic acid, leucine, arginine and aspartate being the predominant amino acids, and the essential amino acid content of micro-endosperm corn protein was 36.15%, which was slightly higher than that of normal corn protein(35.44%). This study can provide a certain scientific basis for the deep processing and application of micro-endosperm corn protein. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
