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| Determination of cyclopropene fatty acids in cottonseed and its cake (meal) by gas chromatography |
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| DOI： |
| KeyWord:cottonseed cyclopropene fatty acids gas chromatography methyl esterification sterculic acid malvalic acid |
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| Author Name | Affiliation | | JIA Yiwen1, CHEN Xiaowei1,SUN Shangde1, BI Yanlan1, CUI Shengcheng2 | 1.College of Food Science and Technology，Henan University of Technology, Zhengzhou 450001, China；2.Xinjiang Taikun Group Co.?, Ltd.?, Changji 831100, Xinjiang, China |
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| Abstract: |
| In order to solve the technical problem of analysis and determination of cyclopropene fatty acids in cottonseed and its cake (meal), gas chromatography (GC) for determination of cyclopropylene fatty acids (malvalic acid and sterculic acid) in cottonseed and its cake (meal) was developed by comparing the methods of fatty acids derivatization and optimizing GC conditions. The results showed that the alkali methyl esterification exhibited a highest efficiency on the derivatization of cyclopropene fatty acids. The optimized GC method required an increasing from 100℃ to 220℃ at a rate of 2.0℃/min at a N2 flow rate of 0.6 mL/min using a DB-1ht capillary column. Thus, the regression equations (R2 ≥ 0.997) of sterculic acid and malvalic acid were established. The contents of malvalic acid in cottonseed, cottonseed cake and cottonseed meal were 2.62, 0.68 mg/g and 0.09 mg/g, and the contents of sterculic acid in cottonseed, cottonseed cake and cottonseed meal were 1.02, 0.27 mg/g and 0.04 mg/g, respectively. The method has good repeatability and recovery, and this finding provides technical basis and guidance for the analysis and determination of cyclopropene fatty acids in cottonseed as well as cottonseed cake and meal. |
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