Factors affecting gossypol content in low temperature cottonseed protein in cottonseed processing line
  
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KeyWord:low temperature cottonseed protein  gossypol content  pretreatment  leaching  drying
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SHAO Hui, HAN Wenjie, ZHU Dandan, LIU Jun, AN Wentao Xinjiang Chenguang Biotechnology Co. , Ltd. , Kashi 844400, Xinjiang, China 
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Abstract:
      In order to reduce the gossypol content in cottonseed protein, combined with production practice, the factors affecting gossypol content in low temperature cottonseed protein in cottonseed processing line were studied and the process of producing low temperature cottonseed protein was optimized. The results showed that the optimal process conditions were obtained as follows: powder degree of cottonseed flake about 10% and moisture content of cottonseed flake about 4% in the pretreatment section; volume fraction of methanol in fresh solvent 92%-93%, volume fraction of methanol in methanol circulating liquid in the first gradient 78%-80%, and 65%-68% in the second gradient, ratio of material mass to fresh methanol volume 1∶ 0.5-1∶ 0.6, methanol circulating liquid temperature 50-53 ℃ and not lower than two-stage gradient countercurrent extraction in the leaching section; as far as possible to extend the drying process time, taking many low temperature drying equipment and gradient continuous drying materials in the drying section. Under the optimal conditions, the gossypol content in low temperature cottonseed protein decreased to less than 400 mg/kg.
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