|
| Effects of ethanol washing pretreatment on camphor seed kernel protein isolate and its protein structure |
| |
| DOI： |
| KeyWord:camphor seed kernel protein ethanol treatment structure solubility |
| FundProject: |
|
| Hits: 943 |
| Download times: 820 |
| Abstract: |
| In order to solve the problem of deep colour and low purity of camphor seed protein, camphor seed kernel protein isolate (CPI) was prepared by alkali solution and acid precipitation method from dehulled, defatted with cold press and crushed camphor seed cake pretreated by different volume fractions of ethanol.The whiteness value, purity, solubility, molecular weight and structure of CPI were analyzed. The results showed that the colour of CPI was obviously improved after 55%-85% ethanol pretreatment. The whiteness value of CPI increased from (0.70±0.02)% to (6.00±0.02)% by 65% ethanol pretreatment. The protein purity of CPI increased from (60.61±0.35)% to (87.45±0.14)% by 95% ethanol pretreatment. After 85% ethanol pretreatment, the solubility of CPI increased from (81.87±0.15)% to (92.12±0.16)%, while after 95% ethanol pretreatment, the solubility decreased to (62.72± 0.05)%. The molecular weight of CPI was mainly distributed in 48, 13 kDa and 35 kDa.After ethanol washing pretreatment, the subunit with the molecular weight of 15 kDa disappeared, and the content of the polymer with the molecular weight of 250 kDa decreased(except that pretreated with 65% ethanol). The hydrogen bonds of protein molecules were destroyed, β-folding and β-turning structure decreased, and random crimp and α-helix structure increased by alkali solution and acid precipitation process, and the secondary structure of CPI was not significantly affected by ethanol. CPI with low colour, good solubility and high purity can be prepared by means of ethanol washing, alkali solution and acid precipitation. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
