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| Extraction optimization and antioxidant interaction kinetics of phenolic compounds from tea seed oil |
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| DOI： |
| KeyWord:tea seed oil phenolic compound extraction by alkaline hydrolysis antioxidant interaction kinetics |
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| Abstract: |
| In order to reveal the antioxidant interaction mechanism of binary phenolic compounds, the phenolic compounds were extracted from tea seed oil by alkaline hydrolysis combined with organic solvent extraction. Through single-factor and orthogonal experiments, the extraction process was optimized, the phenolic compounds composition was analyzed by UPLC-MS/MS, and the scavenge kinetics of DPPH· of 7 phenolic compounds and 21 binary phenolic compounds were studied. The results showed that the optimal extraction conditions of phenolic compounds were obtained as follows: with ethyl acetate as extraction solvent, NaOH concentration 2.0 mol/L, alkaline hydrolysis temperature 45 ℃, alkaline hydrolysis time 5 h, material-liquid ratio 1∶15. Under these conditions, the total phenols content could reach (91.37±1.83)μg/g. A total of 22 phenolic compounds were identified, with total content of 152.181 μg/g, mainly phenolic acids(82.021 μg/g). The compounds of 6,7-dihydroxycoumarin and gentisic acid had a strong synergistic effect. The scavenge rate of binary phenolic compound on DPPH radical was affected by the monomer interaction and the scavenge rate of the phenolic compound which played a major antioxidant role in the compounds. The interaction of phenolic compounds could alter in the process of antioxidation. The storage resistance of tea seed oil is closely related to the antioxidant synergistic effect of phenolic compounds. |
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