| In order to explore the changes of nitrogen-containing substances during sesame seed germination, sesame seeds were soaked respectively in deionized water, 0.5% calcium chloride solution and 1% calcium chloride solution for 30 min and then germinated at 30℃. The changes of crude protein, soluble protein, free amino acids, degree of hydrolysis of protein and molecular weight of protein of sesame seed were measured during 0-72 h germination. The results showed that the content of crude protein decreased first, then increased and then decreased. The soluble protein content increased slightly at 0-4 h, then decreased, and decreased by about 25% at 72 h, and variation trend of three soaking methods was consistent.The content of free amino acids and the degree of hydrolysis of protein increased gradually, and there was significant difference in the content of free amino acids among the three soaking treatments. The results of SDS-PAGE showed that the content of 50-95 kDa protein subunit decreased, the content of 11S protein fluctuated during germination and finally increased, the content of 17 kDa protein subunit increased, and the content of 2S protein decreased significantly. The germination can significantly change the nitrogen content in sesame seeds, macromolecular proteins are degraded into small molecular proteins, and small molecular proteins such as 2S are also degraded. |
