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| Analysis of phospholipids content in dairy products by 31P NMR |
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| DOI： |
| KeyWord:31 P NMR human milk fat infant formula milk powder phospholipid milk fat globule membrane |
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| Author Name | Affiliation | | National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China | National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China |
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| Abstract: |
| In order to determine the content of phospholipids in dairy products, a method for the determination of phospholipid was established based on 31P NMR and the internal standard method was used to quantify the phospholipid. The composition and content of phospholipid in different lactation human milk (colostrum milk, transitional milk, mature milk), milk powder (whole milk powder, infant formula milk powder), whey protein powder rich in lacto-phospholipid, soybean lecithin and egg lecithin were analyzed by this method. The results showed that the limits of detection and quantification of 9 phospholipid standards were 48.97-85.76 μg/mL and 97.94-171.52 μg/mL, respectively. They also demonstrated reasonable recoveries (83.40%-108.52%) and precision (relative standard deviation < 15%). The average total phospholipid content in human milk was 19.27 mg/100 mL, and it decreased gradually with the extension of the lactation period. The composition of phospholipid varied greatly among samples from different sources. Sphingomyelin was the most abundant type in human milk, while phosphatidylcholine was the main type in other samples. |
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