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| Gas chromatographic determination method of trans-linolenic acid with different configurations in linseed oil |
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| DOI： |
| KeyWord:linseed oil trans isomers of α-linolenic acid gas chromatography repeatability detection limit quantification limit linear range |
| FundProject: |
| Author Name | Affiliation | | OU Jinqiang1, SONG Zhihua2, SHAO Linya2, HUANG Jianhua2,WANG Xingguo2, TANG Junjun3 | 1.Wing Biotech.(Jiangsu) Co.,Ltd., Changzhou 213200, Jiangsu, China
2.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
3.Jangsu Xingfumen Grain and Oil Co. , Ltd. , Taizhou 225400, Jiangsu, China |
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| Abstract: |
| In order to quantitatively analyze the cis- and trans- isomers of α-linolenic acid (ALA) in linseed oil, the analytical conditions (carrier gas flow rate and temperature program) for the determination of trans-linolenic acid (TALA) by gas chromatography were optimized on the SLB-IL111 column,and the established method was evaluated. The results showed that the optimal carrier gas flow rate was 0.3 mL/min, and the chromatographic column was initially heated at 60℃ for 5 min, then ramped at 20℃/min to 175℃ and held for 15 min, ramped at 1℃/min to 180℃ and held for 28 min, finally ramped at 0.2℃/min to 185℃ and held for 40 min. The method established had effective separation of 9c,12t,15t-C18∶ 3 and 9t,12c,15t-C18∶3 with a separation degree of 0.9. The separation degree of the other TALA ranged from 1.6 to 8.7. The method was reproducible and the relative standard deviation (RSD) of inter-day repetition of each isomer in heated lineseed oil was 316%-565%, and the RSD of intra-day was 200%-413%.The detection limit was 0.6782-0.8790 mg/L, and the limit of quantification was 2.2606-2.9301 mg/L. The linear relationship between the content of each ALA isomer and the peak area was good, and all the correlation coefficient was 0.9998. In conclusion, the established method can be used for the quantitative analysis of 8 different isomer of ALA in linseed oil. |
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