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| Optimization of enzymatic-assisted preparation of walnut protein and ultrafiltration recovery of walnut protein |
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| DOI： |
| KeyWord:walnut protein cellulase cellulose acetate membrane enzymatic assist ultrafiltration recovery |
| FundProject: |
| Author Name | Affiliation | | GAO Pan1,2,3, ZHANG Huihui1, WANG Shu3,4, YANG Xinmeng1, HU Chuanrong1, HE Dongping1,3, ZHANG Hui5,6, ZHANG Yuejin6, WANG Xingguo1,5,6 | 1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
2.Key Laboratory of Bulk Grain and Oil Intensive Processing, Ministry of Education, Wuhan 430023, China
3.Key Laboratory of Edible Oil Quality and Safety for State Market Regulation, Wuhan 430012, China
4.Wuhan Institute for Food and Cosmetic Control, Wuhan 430012, China
5.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
6.Yunnan MORRE GARDEN Biotechnology Development Co. , Ltd. , Chuxiong 675000, Yunnan, China |
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| Abstract: |
| In order to improve the yield of walnut protein, alkaline protease, cellulase and α-amylase were used as auxiliary enzyme to extract walnut protein by alkali-solution and acid-precipitation, walnut protein from acid precipitation waste water was recovered by ultrafiltration process, and the recovery efficiency of cellulose acetate (CA) membrane, polyethersulfone resin (PES) membrane and polyvinylidene fluoride （PVDF） membrane on walnut protein was compared. The results showed that the optimal preparation conditions of walnut protein were obtained as follows: with cellulase as auxiliary enzyme, enzymatic hydrolysis time 60 min, enzymatic hydrolysis pH 3.6, dosage of enzyme 0.5%, and enzymatic hydrolysis temperature 37℃.Under the optimal conditions,the yield of walnut protein was 84.11%. CA membrane could significantly improve the yield of walnut protein, under the conditions of mass concentration of feed solution 0.363 mg/mL and temperature of feed solution 30℃, the rejection rate of CA membrane could reach 92.33%，and the yield of walnut protein was 87.64%. |
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