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| Preparation, characterization and sustained-release performance of peony seed polypeptide microcapsule |
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| DOI： |
| KeyWord:peony seed antioxidant peptide microcapsules characterization sustained- release performance |
| FundProject: |
| Author Name | Affiliation | | QIAN Senhe1, LI Fangkai1, TU Ximeng1, ZHENG Peng1, WANG Ruonan1, ZHAO Shiguang1,2 | 1.College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000,Anhui，China
2.Xuancheng Industrial Technology Research Institute Co. , Ltd. of Anhui Polytechnic University, Xuancheng 242000, Anhui，China |
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| Abstract: |
| Based on the analysis of antioxidant activity of peony seed polypeptide, the peony seed polypeptide microcapsules were prepared by sharp hole coagulation method to improve the stability of peony seed polypeptide. Taking the embedding rate as the index, the mass fractions of CaCl2 and sodium alginate, core-wall ratio and reaction temperature in the preparation of peony seed polypeptide microcapsules were optimized by single factor experiment and response surface experiment. Meanwhile, the peony seed polypeptide microcapsules were characterized and the sustained-release performance were analyzed.The results showed that the peony seed polypeptide had good scavenging capacity for hydroxyl radical, ABTS+ radical and DPPH radical. The optimal preparation conditions of microcapsules were obtained as follows: CaCl2 mass fraction 2.20%, sodium alginate mass fraction 1.80%, core-wall ratio 1∶3, and reaction temperature 52℃. Under these conditions, the embedding rate was 83.17%. The infrared spectrum of peony seed polypeptide microcapsule was red shifted compared with peony seed polypeptide, and the absorption intensity was less than that of peony seed polypeptide. Scanning electron microscopy showed that the size of peony seed polypeptide microcapsule was about 0.7 mm, and there were pits and cracks on its surface under low power microscope and many folds under high power microscope. Peony seed polypeptide microcapsules could exist stably in artificially simulated gastric juice and release slowly in artificially simulated intestinal juice, and the release rate reached 95.43% after 3 h. |
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