In vitro bioaccessibility of fatty acid stigmasterol esters with different saturation
  
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KeyWord:phytosterol ester  fatty acid saturation  in vitro digestion  bioaccessibility
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Author NameAffiliation
WANG Tao1, LIU Ruijie1, WANG Yanjun2, WANG Xingguo1, CHANG Ming1 1.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China2.Bayannur City Science and Technology Achievement Transformation Center, Bayannur 150800, Inner Mongolia, China 
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Abstract:
      In order to investigate the effect of fatty acid saturation on the bioaccessibility of phytosterol ester, a static model of in vitro digestion was used to study the digestion characteristics of stigmasterol oleate, stigmasterol linoleate and stigmasterol linolenate. The results showed that after the simulated digestion in vitro (120 min), the free fatty acid (FFA) release rate of stigmasterol linoleate was the highest with 78.51%, followed by stigmasterol linolenate with 74.21%, and that of stigmasterol oleate was the lowest with 69.15%. The hydrolysis rates of the three stigmasterol esters decreased in the order: stigmasterol linolenate > stigmasterol linoleate> stigmasterol oleate. The degree of hydrolysis of stigmasterol esters decreased in the order: stigmasterol linoleate > stigmasterol linolenate > stigmasterol oleate. The bioaccessibilities of stigmasterol oleate, stigmasterol linoleate and stigmasterol linolenate were 14.01%, 11.11% and 10.19% respectively, and the overall difference was small. In conclusion, the degree of saturation of bound fatty acids (same carbon chain length) in stigmasterol esters affects the hydrolysis rate, but has little effect on the bioaccessibility of stigmasterol esters.
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