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| Process optimization and mechanism of hydrolysis of soybean oil catalyzed by lipase from Candida rugosa |
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| KeyWord:lipase from Candida rugosa soybean oil fatty acid hydrolysis mechanism |
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| Abstract: |
| In order to reduce the application cost of lipase catalyzed hydrolysis of vegetable oil, the lipase from Candida rugosa(CRL) was used as a catalyst, and the effect of hydrolysis of different vegetable oils with CRL was compared. Selecting soybean oil as the research object, the influence of pH, reaction temperature, reaction time, volume ratio of water to oil and dosage of enzyme on hydrolysis rate was investigated by single factor experiment, then the response surface methodology was applied to optimize the process conditions. The results indicated that the selectivity of hydrolysis of C18 fatty acid glyceride catalyzed by CRL was higher. The optimal process conditions of hydrolysis of soybean oil catalyzed by CRL were obtained as follows: reaction temperature 39.5℃, pH 6.7, volume ratio of water to oil 1∶2.2, and enzyme dosage 0.6% (based on soybean oil mass). Under these conditions, the hydrolysis rate achieved 77.21% within 2 h and 96.33% within 12 h. The main reaction mechanism of CRL catalyzed hydrolysis of triglycerides was serine in the nucleophilic catalytic triad reacted nucleophilically with the carbonyl group of triglyceride to seize the fatty acid precursor, and then water molecule reacted nucleophilically with the precursor to release fatty acid. CRL can efficiently catalyze the production of fatty acids from vegetable oils under mild conditions, and it is a biocatalyst with broad application prospects. |
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