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| Genome identification and functional analysis of a Bacillus subtilis strain efficiently degrading glucosinolates in rapeseed meal |
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| DOI： |
| KeyWord:rapeseed meal glucosinolates comparative genomics Bacillus subtilis |
| FundProject: |
| Author Name | Affiliation | | XIONG Chuan1, LUO Qiang2, LI Ping1, JIN Xin1, ZHANG Juan3, ZHANG Li4, ZHU Yu1 | 1.Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, China
2.Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education),The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
3.Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
4.Institute of Forestry and Grassland Science and Technology of Aba Prefecture, Sichuan Province, Wenchuan 623000, Sichuan,China |
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| Abstract: |
| In order to screen bacteria that can efficiently degrade glucosinolates in rapeseed meal and provide experimental data for the construction of glucosinolate-degrading genetically engineered bacteria, the soil stacked with rapeseed meal was used as the separation source, and a strain C1 with the greatest glucosinolate degradation rate was obtained by glucosinolate selection medium screening and glucosinolate degradation rate determination,and 16S rRNA gene sequence was used for C1 species identification and the third generation Illumina NovaSeq PE150 was used to sequencing the whole genome to obtain C1 genome completion map and functional annotation was carried out. The comparative genomics was used to explore the mechanism for glucosinolate degradation of strain C1. The results showed that strain C1 was Bacillus subtilis, and its glucosinolate degradation rate was (68.98±4.74)%. The genome chromosome of the strain C1 was 4 139 381 bp in length, and the guanine and cytosine content was 43.88%. The total length of the coding region accounted for 89.14% of the whole genome. Strain C1 could encode a large number of functional proteins, mainly related to amino acid transport and metabolism. Comparative genomics analysis confirmed that C1 was highly colinear with the model strain of Bacillus subtilis (NC000964.3), and there were no large fragment translocation and inversion regions in the sequence. However, a small fragment insertion was found at 2.7 Mb in C1 sequence, and a small fragment deletion appeared at 2.1-2.4 Mb,covering two gens of myrosinase, respectively, and the mechanism of glucosinolate degradation of C1 was presumed to efficiently secrete myrosinase. Strain C1 can efficiently degrade glucosinolates in rapeseed meal and is a potential material for constructing glucosinolate-degrading genetically engineered bacteria. |
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