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| Advances in biosynthesis of microbial oils in metabolically engineered Yarrowia lipolytica |
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| DOI： |
| KeyWord:Yarrowia lipolytica microbial oil metabolic engineering synthetic biology |
| FundProject: |
| Author Name | Affiliation | | SHEN Yuping1,2, CHEN Shijie1, ZHOU Xun1, ZHANG Zujiao1,2 | 1.College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, Hunan, China
2.Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou 425199, Hunan, China |
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| Abstract: |
| Microbial oils are an important direction for the development of renewable energy. In recent years, the oils production of engineered Yarrowia lipolytica has been rapidly improved through synthetic biology methods and metabolic engineering technology, showing an attractive application prospect. The latest research advances in oils production by Y. lipolytica modified by metabolic engineering were summarized as the follow eight aspects: regulation of key enzymes of metabolic pathways, relief of negative feedback regulation, heterologous expression of key enzymes of metabolic pathways, construction of acetyl-CoA and NADPH alternative pathways, protection against oxidative stress, enhancement of fatty acid secretion, adaptive evolution and computer-assisted simulation. The existing research showed that the toxic components in cheap substrates affecting cell growth and oil synthesis as well as the incomplete clarity of the mechanism of oils regulatory network was the bottleneck restricting the improvement of oils production in Y. lipolytica. Therefore, designing and introducing detoxification pathways, adding antidotes, or screening strain tolerant to toxic compound, as well as further elucidating metabolic regulatory mechanisms using multi-omics techniques and computer-assisted can address this issues. In addition, in a ″ double-carbon″ background, efficient artificial photosynthesis or carbon fixation pathways can be introduced in Y. lipolytica to produce oils with carbon dioxide. |
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