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| Determination of 16 polycyclic aromatic hydrocarbons in oil-tea camellia seed oil using molecularly imprinted solid-phase extraction coupled with GC-MS/MS |
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| DOI： |
| KeyWord:oil-tea camellia seed oil polycyclic aromatic hydrocarbons molecularly imprinted solid-phase extraction GC-MS/MS |
| FundProject: |
| Author Name | Affiliation | | ZHONG Donglian1,YU Ninghua2,WANG Meijuan1, MO Ｒunhong1, DUAN Junmin2, TANG Fubin1 | 1.Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2.Hunan Quality Inspection and Testing Center for Forestry Products, Changsha 410004, China |
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| Abstract: |
| In order to analyze polycyclic aromatic hydrocarbons(PAHs) in oil-tea camellia seed oil stably and reliably, a method for the determination of 16 PAHs in oil-tea camellia seed oil was developed by molecularly imprinted solid-phase extraction(MIP-SPE)-gas chromatography-tandem mass spectrometry(GC-MS/MS). The samples were dissolved by n-hexane, then purified by reversed-phase solid-phase extraction HLB column, Florisil column and MIP-SPE column, respectively, and the recovery and matrix effect were used as the investigation indexes to evaluate the purification effect. On this basis, the purification solution was concentrated by low-temperature and low-speed nitrogen blowing, detected by GC-MS/MS in multiple reaction monitoring scan mode, and quantified by external standard method.The results showed that the MIP-SPE column had the best purification effect on 16 PAHs.The linearity of the 16 PAHs was good in the range of mass concentration from 1 μg/L to 50 μg/L (R2≥0.995 1), and the limits of detection (LODs) range from 0.01 μg/kg to 0.20 μg/kg. The recoveries varied from 71.5% to 116.3% with relative standard deviation of 1.5% to 13.8% at spiked levels of 2, 10 μg/kg and 20 μg/kg. The method has the characteristics of high sensitivity, low detection limit and good precision, and is suitable for rapid and accurate quantitative detection of 16 PAHs in oil-tea camellia seed oil. |
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