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| Simultaneous determination of eight vitamin E isomers in vegetable oils by RP-HPLC |
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| DOI： |
| KeyWord:RP-HPLC homogeneous rapid extraction vegetable oil vitamin E |
| FundProject: |
| Author Name | Affiliation | | WU Limei1, WU Lihua1,2, ZHAO Jingjing2, WU Xiaoxia1, LI Jianke1 | 1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi′an 710119, China
2.Xi′an Grain & Oil Quality Inspection Center, Xi′an 710003, China |
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| Abstract: |
| In order to accurately and quickly determine eight vitamin E isomers in vegetable oils，a method for simultaneous determination of eight vitamin E isomers in vegetable oils by homogeneous rapid extraction combined with RP-HPLC was established, and the method was used to determine 14 common vegetable oils. The results showed that compared with the conventional saponification method and solid-phase extraction method, the homogeneous rapid extraction method was fast and simple, with high accuracy, precision and good reproducibility. Eight kinds of vitamin E isomers could be well separated within 15 min. The linearities of eight vitamin E isomers were well in the range of 0.5-10 μg/mL(R2>0.999). The detection limits, quantitation limits, relative standard deviations and spiked recoveries were 0.02-0.05 μg/mL, 0.07-0.17 μg/mL, 2.48%-5.79% and 90.00%-110.00%, respectively. There were obvious differences in the types and contents of tocopherols and tocotrienols in different edible vegetable oils. The established homogeneous rapid extraction combined with RP-HPLC is suitable for the simultaneous determination of eight vitamin E isomers in vegetable oils. |
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