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| Antioxidant activity of different molecular modified products of polysaccharide from Camellia oleifera seed meal |
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| KeyWord:Camellia oleifera seed meal polysaccharide molecular modification antioxidant activity |
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| Abstract: |
| In order to compare the effects of different modification methods on the antioxidant activity of polysaccharide from Camellia oleifera seed meal, the purified polysaccharides from Camellia oleifera seed meal (COP) was molecularly modified by sulfation, carboxymethylation and acetylation to prepare COP with different degrees of substitution. Using an in vitro test method, the antioxidant activity of COP and its molecular modification products was investigated with the scavenging rate of hydroxyl radical (·OH), superoxide anion radical (·O-2) and DPPH radical (DPPH·) as indicators. The results showed that the sulfation modification with low degree of substitution could improve the scavenging ability of COP on ·OH, ·O-2 and DPPH·. Carboxymethylation with high degree of substitution could improve the ability of COP to scavenge ·OH, and carboxymethylation with low degree of substitution could increase the ability of COP to scavenge DPPH·. The acetylated modified COP with high degree of substitution had better scavenging effect on ·OH and DPPH·. Carboxymethylation and acetylation could reduce the ability of COP to scavenge ·O-2. In conclusion, moderate molecular modification can improve the antioxidant activity of COP. |
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