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| Synthesis of high purity propylene glycol monoesters from oil-tea camellia seed oil catalyzed by mono- and diacylglycerol lipases |
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| DOI： |
| KeyWord:propylene glycol monoester oil-tea camellia seed oil mono- and diacylglycerol lipase enzymatic catalysis |
| FundProject: |
| Author Name | Affiliation | | IU Zhuang1, LIU Xuan1, LUO Riming2, CHEN Dongsheng3, YANG Bo4, WANG Weifei1,LAN Dongming1 | 1.College of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, China
2.Guangdong YUE-SHAN Special Nutrition Technology Co. , Ltd. , Foshan 528226, Guangdong,China
3.COFCO ET (Xi′an) International Engineering Co. , Ltd. , Xi′an 710082, China
4.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510640, China |
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| Abstract: |
| To improve the proportion of monoesters in the synthesis of propylene glycol esters, the synthesis of propylene glycol monoesters was catalyzed by the esterification of mono- and diacylglycerol lipases Lipase AOL and Lipase G Amano 50 with oil-tea camellia seed oil fatty acids as raw materials. The effects of enzyme addition, reaction temperature, substrate molar ratio and rotational speed on the synthesis of propylene glycol monoesters by esterification reaction were investigated, and the products were identified and compared with Lipase SMG1-F278N catalyzed esterification. The results showed that the optimal reaction conditions for the synthesis of propylene glycol monoesters by Lipase AOL and Lipase G Amano 50 were as follows: Lipase AOL with 80 U/g of addition and 1∶4 molar ratio of oil-tea camellia seed oil fatty acids to 1,3- propylene glycol, Lipase G Amano 50 with 100 U/g of addition and 1∶2 molar ratio of oil-tea camellia seed oil fatty acids to 1,3- propylene glycol, reaction temperature 35℃, reaction time 18 h, and rotational speed 180 r/min. Under these conditions, the contents of propylene glycol monoesters reached 85.55% and 72.98% by Lipase AOL and Lipase G Amano 50 catalyzed esterification. The synthesized propylene glycol monoesters were identified by GC-MS as consisting of propylene glycol monopalmitate, propylene glycol monolinoleate and propylene glycol monooleate. Compared with Lipase G Amano 50 and Lipase SMG1-F278N, Lipase AOL catalyzed the synthesis of propylene glycol monoesters with higher content and purity, and it was more suitable as an enzyme for the catalytic synthesis of propylene glycol monoesters. |
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