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| Nutrient components,optimization of oil extraction conditions and fatty acid composition analysis of Tianshan cold water algae |
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| DOI： |
| KeyWord:Tianshan cold water algae protein polysaccharide chlorophyll oil fatty acid |
| FundProject: |
| Author Name | Affiliation | | ASKAR Tursun1, RUXIANGULI Maimatyiming1, GULIFEIZHE Yilihamu1, XIAO Yicong1, PAN Hongjun1，AIMAITI Mematim2，ZULIHUMAER Yakupu1，GULIJIANAITI Abula1，AIHAMATIJIANG Aihaiti1, QIN Xinzheng3 ，LIU Jun1 | 1.Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
2.Xinjiang Xinguang Biological Technology Co. , Ltd. , Urumqi 830000, China
3.Institute of Microbiology Application, Xinjiang Academy of Agricultural Sciences, Urumqi 830000,China |
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| Abstract: |
| In order to provide reference data for research on the efficacy of Tianshan cold water algae, using the Tianshan cold water algae powder from Sayram Lake in Xinjiang as raw materials, the Kjeldahl method was used to determine its protein content，the polysaccharide content was determined by the phenol-sulfuric acid method, the chlorophyll content was determined by spectrophotometric colorimetry, the oil was extracted by ultrasonic-assisted organic solvent extraction method, and the oil extraction conditions were optimized by single-factor experiment, and the fatty acid composition and content of the oil were determined by gas chromatography. The results showed that the protein content of Tianshan cold water algae was 41.58%, the polysaccharide content was 15.34%, and the total chlorophyll content was 2.42%. The optimal extraction conditions of Tianshan cold water algae oil were 70% ethanol as the extraction solvent, solid-liquid ratio 1∶25, and ultrasonic extraction time 20 min. Under these conditions, the oil yield of Tianshan cold water algae was 32.80%. A total of 14 fatty acids were detected in Tianshan cold water algae oil, mainly polyunsaturated fatty acids, accounting for 66.53% of the total fatty acids, mainly including linoleic acid, α-linolenic acid and arachidonic acid, with contents of 50.77%,13.28% and 2.48% respectively. The contents of protein, polysaccharide and chlorophyll in Tianshan cold water algae are relatively high, and the polyunsaturated fatty acid content in its oil is also relatively high, which makes Tianshan cold water algae have great research value. |
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