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| Determination of four heterocyclic aromatic amines in vegetable oils by ultra-performance liquid chromatography-tandem mass spectrometry |
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| DOI： |
| KeyWord:heterocyclic aromatic amines UPLC-MS/MS vegetable oil |
| FundProject: |
| Author Name | Affiliation | | REN Dandan1, CHEN Xiuhong2, SUN Dongzhe1, WANG Manyi1, YU Lei3, WANG Xiangyu1 | 1.COFCO Nutrition and Health Research Institute, Beijing Key Laboratory of Nutrition Health and Food Safety, Beijing Engineering Laboratory of Geriatric Nutrition & Foods, Beijing 102209, China
2. International Trade Food Science and Technology (Beijing) Co. , Ltd. ， Beijing 102209, China
3. COFCO Oils Specialized Company, Beijing 100020, China |
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| Abstract: |
| A method for determination of four heterocyclic aromatic amines(MeIQx, PhIP, 4,8-DiMeIQx and 7,8-DiMeIQx) in vegetables oils by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples were extracted by acetonitrile containing 1% ammonium hydroxide, defatted by n-hexane saturated by acetonitrile, followed by the purification with PCX solid phase extraction column. Then the heterocyclic aromatic amines in the extracts were separated by a gradient elution of acetonitrile and 10 mmol/L ammonium formate aqueous solution, then quantitative and qualitative ion of heterocyclic aromatic amines were monitored by electrospray ionization tandem mass spectrometry in positive ion mode, and quantified by internal standard method. The results showed that all of the analytes showed good linearity in the range of 0.5-100.0 ng/mL and the correlation coefficients (r2) were all above 0.99. The limits of detection for heterocyclic aromatic amines were 0.01-0.04 μg/kg, and the limits of quantification were 0.03-0.13 μg/kg. The average recoveries of heterocyclic aromatic amines at spiked levels of 1.0, 5.0, 10.0 μg/kg ranged from 64.5% to 96.5%, with the relative standard deviations of 2.83%-8.52%. The method is rapid, accurate and sensitive, and is suitable for the determination of four heterocyclic aromatic amines in vegetable oils. |
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