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| Optimization of bioenzyme-catalyzed methyl esterification of deodorized distillates by response surface methodology |
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| DOI： |
| KeyWord:bioenzyme deodorized distillates methyl esterification |
| FundProject: |
| Author Name | Affiliation | | WU Wenhua1, GUAN Guohua1, JIANG Yiming1, SONG Lianfang2, WANG Kuan2, GENG Xinqi2, DENG Jun2, XU Wei2, XU Xiaoran1, TONG Qiang1 | 1.COFCO TECH Bioengineering (Tianjin) Co. , Ltd. , Tianjin 300457, China
2.COFCO Biotechnology Co. , Ltd. , Bengbu 233010, Anhui, China |
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| Abstract: |
| In order to realize the green, clean and efficient methyl esterification of deodorized distillates and achieve the goal of environmental protection, the reaction conditions of the bioenzyme-catalyzed methyl esterification of deodorized distillates were optimized by single-factor experiment and response surface methodology, and the changes of vitamin E content before and after the reaction were measured. In addition, the new process was applied to the actual production line. The results showed that the optimal reaction conditions for the methyl esterification of deodorized distillates catalyzed by bioenzyme were as follows: water dosage 1%(based on the mass of deodorized distillates), methanol dosage 11.5% (based on the mass of deodorized distillates), immobilized lipase dosage 0.3% (based on the mass of deodorized distillates), reaction temperature 35.6℃. Reaction under the optimal conditions for 8 h, the esterification rate of free fatty acid and glycerol ester in the deodorized distillates reached 92.59% and 94.95%, respectively. In the methyl esterification of the bioenzyme-catalyzed deodorized distillates, the loss of vitamin E was low, and its yield reachedg 93%. The total yield of vitamin E (50% vitamin E) increased by 3.47 percentage points and the total yield of phytosterols increased by 3.26 percentage points compared with the concentrated sulfuric acid catalyzed methyl esterification process. In conclusion, the bioenzyme-catalyzed methyl esterification of deodorized distillates posses higher esterification rates and yields of vitamin E and phytosterols. |
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