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| Extraction and antioxidant activity of polysaccharide from Moringa oleifera seed by internal boiling method |
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| DOI： |
| KeyWord:Moringa oleifera seed polysaccharide internal boiling method antioxidant activity |
| FundProject: |
| Author Name | Affiliation | | HU Linghua1 , NIU Yanfei1,2， TIAN Ruifeng1 , WANG Bin1 , CHEN Baosen3 , WU Chunhua1 | 1. School of Chemistry and Engineering, Southwest Forestry University, Kunming 650224,China
2. Yunnan Institute of Materia Medica, Kunming 650111,China
3. Yunnan Weedtechnology Biotechnology Co. , Ltd. , Yiliang 652100, Yunnan,China |
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| Abstract: |
| In order to accelerate the extraction rate of Moringa oleifera seed polysaccharide, the polysaccharide was extracted from Moringa oleifera seed by internal boiling method. Single factor experiment and response surface methodology were used to optimize the internal boiling extraction conditions of Moringa oleifera seed polysaccharide, and the antioxidant activity of free radical scavenging activity was studied. The results showed that the optimal extraction conditions were obtained as follows:volume fraction of desorbent ethanol 20%, distilled water extraction temperature 74℃, extraction time 5 min, and solid-liquid ratio 1∶27. Under the optimal conditions, the yield of Moringa oleifera seed polysaccharide could reach 12.5%. The results of antioxidant activity study showed that the hydroxyl free radical scavenging rate of Moringa oleifera polysaccharide with a mass concentration of 5.0 mg/mL was 91.1%. The DPPH scavenging rate of Moringa oleifera polysaccharide with a mass concentration of 1.0 mg/mL was 38%. Superoxide anion scavenging rate of Moringa oleifera polysaccharide with a mass concentration of 5.0 mg/mL was 59.8%. In conclusion, Moringa oleifera seed polysaccharide has some antioxidant activity. |
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