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| Extraction of borage seed oil and its effect on antioxidant enzyme activity of skeletal muscle in exhaustive exercise rats |
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| KeyWord:borage seed oil exhaustive exercise skeletal muscle antioxidant enzyme |
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| Abstract: |
| In order to promote the development and utilization of borage seed oil, taking borage seed as raw material, the borage seed oil was extracted with the subcritical butane extraction method. The effects of liquid-material ratio, extraction temperature and extraction time on the extraction rate of borage seed oil were studied by single factor experiment. The extraction conditions of borage seed oil were optimized by response surface methodology. The fatty acid composition of the borage seed oil was analyzed. In addition, SD rats were divided into five groups on average. The control group did not receive training, and the remaining four groups received exhaustive exercise training. The low, medium and high dose groups were gavaged with 10, 15 mg/g and 20 mg/g borage seed oil every day, the exhaustive exercise group was gavaged with 15 mg/g distilled water every day, and the control group drank water normally. After the experiment, the activity of antioxidant enzymes in the skeletal muscle of the rats was determined to investigate the effect of borage seed oil on the activity of antioxidant enzymes in the skeletal muscle of exhausted exercise rats. The results showed that the optimum extraction conditions of borage seed oil were as follows: liquid-material ratio 6.2∶1, extraction temperature 41℃, extraction time 53 min. Under these conditions, the extraction rate of borage seed oil was 35.32%. The content of unsaturated fatty acids in borage seed oil was 85.51%. The borage seed oil could restore the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in skeletal muscle, and reduce the content of malondialdehyde (MDA) in skeletal muscle. Borage seed oil can increase the activity of antioxidant enzymes in skeletal muscle of exhausted exercise rats, improve the body′s ability to scavenge free radicals and reduce the damage to body cells. |
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