| To explore the enzymatic catalytic synthesis process of medium long-chain fatty acid triglyceride (MLCT)with high MLCT content and low cost, lipase Lipozyme TL IM enzymatic catalytic transesterification was used to synthesize MLCT using first-grade rapeseed oil as raw material, and the effects of substrate ratio(mass ratio of rapeseed oil to medium chain triglycerides), reaction time, reaction temperature and enzyme dosage on transesterification reaction were investigated by single factor experiment. Then the synthesis process conditions were optimized by orthogonal experiment. The results showed that the optimal synthesis process conditions were obtained as follows: substrate ratio 3∶1, reaction time 4 h, reaction temperature 50℃, and enzyme dosage 8%(based on the mass of substrate), and the MLCT content reached 87.50% under the optimal conditions. The optimized process has advantages of high MLCT content, short reaction time and low reaction temperature, which can reduce energy consumption and enzyme inactivation, thus reducing the production cost. |
