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| Protein components and activity of endogenous proteases in pecan kernels |
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| DOI： |
| KeyWord:pecan kernel protein antioxidant enzymes endogenous proteases |
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| Author Name | Affiliation | | ZHOU Qianqian1, YU Wang1, MU Wanmeng2, CHEN Yeming1 | 1.Research Center of Lipid & Vegetable Protein, Jiangnan University, Wuxi 214122, Jiangsu, China
2.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China |
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| Abstract: |
| In order to systematically examine the major proteins in pecan kernels, especially the bioactive proteins with low relative abundance, the major proteins and bioactive proteins(antioxidant enzymes, endogenous proteases) in fresh pecan kernels were investigated by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography tandem mass spectrometry and amino acid analysis.The optimum pH and temperature of the endogenous proteases were also studied. The results showed that the major proteins in pecan kernels included 11S globulins (58%), 7S globulins (17%) and 9 kDa proteins (15%), as well as 0.239% superoxide dismutases, 0.190% catalases and 0.282% endogenous proteases. Only one protease inhibitor was detected, and its relative abundance was extremely low. These endogenous proteases showed hydrolytic activity at pH 3.5-5.5 and 40-60℃, and optimal at pH 4.5 and 50℃. As for the amino acid composition, the proteins in pecan kernels contained a low content of lysine (3.74%), but a high content of glutamic acid + glutamine (19.51%) and arginine (14.12%). It is suggested that domestic pecans are suitable for fresh consumption, which is benifical for promoting human health. |
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