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| Extraction and amino acid composition analysis of zein from micro-endosperm maize |
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| DOI： |
| KeyWord:micro-endosperm maize zein ethanol extraction method ultrasonic assisted ethanol extraction method amino acid composition |
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| Abstract: |
| To promote the development and application of zein from micro-endosperm maize, zein was extracted from micro-endosperm maize by ethanol extraction method and ultrasonic assisted ethanol extraction method. The process conditions were optimized by single factor experiments, and the amino acid composition of zein from micro-endosperm maize was analyzed. The results showed that the optimal conditions for ethanol extraction method were obtained as follows: extraction temperature 50℃, extraction time 2.0 h, ethanol volume fraction 80% and solid-liquid ratio 1∶12. The optimal conditions for ultrasonic assisted ethanol extraction method were obtained as follows: ethanol volume fraction 80%, ultrasonic time 40 min, solid-liquid ratio 1∶20 and ultrasonic power 500 W. Under these optimal conditions, the extraction rate and protein content of zein from micro-endosperm maize extracted by ultrasonic assisted ethanol extraction method were 45.88% and 82.1%, respectively, which were significantly better than 32.61% and 79.2% extracted by ethanol extraction method. The non-polar amino acid proportion of zein from micro-endosperm maize was 59.11%, which was higher than 53.38% of common zein. In conclusion, compared with ethanol extraction method, ultrasonic assisted ethanol extraction method has stronger advantages, and zein from micro-endosperm maize shows stronger hydrophobicity than common zein. |
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