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| Ultrafiltration membrane classification of zein peptide and the in vitro biological activities of the product |
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| DOI： |
| KeyWord:zein zein peptide ultrafiltration classification molecular weight antioxidant activity |
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| Abstract: |
| In order to study the effect of molecular weight on the in vitro biological activities of zein peptide, zein was hydrolyzed by alkaline protease Alcalase to prepare zein peptide, and then the zein peptide was fractionated sequentially using ultrafiltration membranes with molecular weight cut-off 5,3 ku and 1 ku, and four components with molecular weight more than 5 ku, 3-5 ku, 1-3 ku and less than 1 ku were obtained. The effect of molecular weight on the biological activities of zein peptide was characterized by measuring the antioxidant and alcohol dehydrogenase activation activities of each molecular weight component. The results showed that when zein was hydrolyzed with alkaline protease Alcalase for 2 h, the molecular weight of the zein peptide that had the ability to scavenge DPPH free radicals was in the range of 1-5 ku. The molecular weight of the zein peptide with the ability to chelate ferrous ions, scavenge hydroxyl free radicals and activate alcohol dehydrogenase was concentrated less than 1 ku. However, the superoxide anion radical scavenging activity and reducing power of zein peptide were less affected by the molecular weight. In conclusion, the biological activities of zein peptide were greatly affected by the molecular weight, and ultrafiltration membranes could be used to fractionate and concentrate the active components of zein peptide. |
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