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Preparation of high-purity phosphatidylethanolamine by freezing crystallization
  
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KeyWord:soybean phospholipids  phosphatidylethanolamine  freezing crystallization
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SHEN Xiaohua, CAO Dong School of Food Science and Technology，Jiangnan University，Wuxi 214122, Jiangsu, China 
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Abstract:
      To solve the backlog problem of soybean phospholipids with low phosphatidylcholine(PC)content in factories and to obtain high-purity phosphatidylethanolamine (PE), the crude PE product extracted from soybean phospholipids with low PC content by alkaline ethanol was used as the raw material to prepare high-purity PE by freezing crystallization. Based on the single factor experiment, with PE content as the indicator, the optimal freezing crystallization conditions of PE were determined by response surface methodology as follows: solid liquid ratio 1∶40, freezing time 21.5 h, freezing temperature -25℃,volume fraction of ethanol 94%, ethanol alkali concentration （volume ratio of 94% ethanol to 25% ammonia water） 100∶8. Under these conditions, the content and recovery rate of PE were 76.56% and 81.25%, respectively. Therefore, the freezing crystallization process can be used to prepare high-purity PE.
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