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| QuEChERS-超高效液相色谱法测定食用油中5种抗氧化剂 |
| Determination of five antioxidants in edible oil by QuEChERS-ultra high performance liquid chromatography |
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| DOI： |
| 中文关键词: QuEChERS 超高效液相色谱 食用油 抗氧化剂 |
| 英文关键词:QuEChERS ultra high performance liquid chromatography edible oil antioxidant |
| 基金项目: |
| Author Name | Affiliation | | LI Ya1, LIANG Jianfeng1,KE Lijian2, ZHONG Shuiqiao2,JIANG Deli2 | 1.College of Food and Pharmaceutical Engineering,Wuzhou University, Wuzhou 543002， Guangxi, China
2.Wuzhou Food and Drug Inspection Institute, Wuzhou 543002, Guangxi, China |
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| 中文摘要: |
| 为快速准确测定食用油中抗氧化剂含量，通过QuEChERS-超高效液相色谱法建立同时测定食用油中丁基羟基茴香醚（BHA）、特丁基对苯二酚(TBHQ)、二丁基羟基甲苯(BHT)、没食子酸丙酯(PG)、没食子酸辛酯（OG）5种常见抗氧化剂含量的方法。样品先采用甲醇溶解提取，提取液经冷冻离心、净化剂净化分离后上机检测，超高效液相色谱仪以乙腈-水为流动相进行梯度洗脱，采用外标法定量。结果表明：5种抗氧化剂在1.0~100.0 mg/L范围内线性关系良好，检出限均不大于1.6 mg/kg，加标回收率在85.78%~100.85%之间，RSD在2.40%~6.01%之间。综上，该方法具有操作简单、重现性好、灵敏度高等特点，适合动植物油中5种常用抗氧化剂含量的检测与分析。 |
| 英文摘要: |
| In order to quickly and accurately determine the antioxidants in edible oil, a method for simultaneous determination of butylhydroanisole(BHA), tertiary butylhydroquinone (TBHQ), dibutylhydrotoluene (BHT), propyl gallate (PG) and octyl gallate (OG) in edible oil was established by QuEChERS-ultra high performance liquid chromatography.The sample was dissolved and extracted with methanol, and the extract was tested on the machine after freezing centrifugation and purification and separation with detergent. The ultra high performance liquid chromatography used acetonitrile-water as the mobile phase for gradient elution, and the external standard method was used for quantification. The results showed that the linear relationship of the five antioxidants was good in the range of 1.0-100.0 mg/L, the detection limits were not more than 1.6 mg/kg, the spiked recovery rate was 85.78%-100.85%, and the RSD was 2.40%-6.01%. In conclusion, this method has the characteristics of simple operation, good reproducibility and high sensitivity, and is suitable for the detection and analysis of common antioxidants in animal and vegetable oils. |
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